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Online edition:ISSN 2434-3404

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Haplotypes of the /?E-Globin Gene Cluster Found in Myanmar

We had the opportunity to study the members of 10 families with Hb E//?-thalassemia and characterized the ethnic features of the population of Myanmar from haplotyping of a /?-globin-like gene cluster producing Hb E. Detection of the Hb E and /?E-globin genes was carried out by DEAE-HPLC and isoelectric focusing of the hemolysates, by electrophoresis of DNA fragments which resulted in the digestion of the PCR product with Mnll, and by PCR-direct sequencing. The haplotype of the /3-globin-like gene cluster was determined by the presence (+) or absence (-) of the restriction enzyme site in the PCR product including the polymorphic site. Polymorphism or the haplotype were determined at seven sites, 5' to the 」>gene//JmcII, in the ー7-gene/ifmdIII, in the Ay-gene/#mdIII, in the

Author
HARANO K, et al
Volume
29
Issue
1-2
Pages
9-16
DOI
10.11482/KMJ29(1.2)9-16.2003.PDF.pdf

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