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Online edition:ISSN 2434-3404

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Use of Pronase and Pepsin in the Restoration of Laminin Antigenicity

To improve the restoration of laminin antigenicity, we applied pronase E and pepsin before immunohistochemical staining. In changing the concentration and reaction times of these enzymes, we found that the best results were obtained in sections treated with a 120 min pepsin reaction followed by 5 to 10 min pronase E treatment. Constant and consistent results were obtained with this procedure, and it is expected to improve the immunohistochemical study of laminin.

Author
Yoshida K, et al
Volume
19
Issue
3.4
Pages
101-105
DOI
10.11482/KMJ19(3.4)101-105.1993.pdf

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