Identification and analysis of FANCD2-interacting factors *
Fanconi anemia（FA）is a rare hereditary disorder, clinically characterized by progressive bone marrow failure, cancer susceptibility, and genome instability. FA cells display hypersensitivity to interstrand DNA crosslinking agents, such as cisplatin and mitomycin C（MMC）. Eleven of the FA genes（FANCA, B, C, D1, D2, E, F, G, J, L and M）have been identified at present. These genes have been considered to cooperate in a common DNA repair pathway（the FA pathway）. Among them, eight FA gene products（FANCA, B, C, E, F, G, L and M）form the FA core complex, which monoubiquitinates FANCD2, a central molecule of the FA pathway following DNA damage. However, the function of FANCD2 remained unknown. To gain insight into its function and regulatory mechanisms, we isolated 33 clones by using the Yeast Two-hybrid method as an interaction factor of chicken FANCD2. We selected BCCIP and DEADC1 as likely interacting factors, and tried to delete both genes in the chicken B cell line DT40, but neither knockout cell line could be obtained. These results suggest that both genes are essential for cell viability. In the future, it will be necessary to analyze them by methods such as siRNA.（Accepted on October 17，2006）
