Haplotypes of the /?E-Globin Gene Cluster Found in Myanmar
We had the opportunity to study the members of 10 families with Hb E//?-thalassemia and characterized the ethnic features of the population of Myanmar from haplotyping of a /?-globin-like gene cluster producing Hb E. Detection of the Hb E and /?E-globin genes was carried out by DEAE-HPLC and isoelectric focusing of the hemolysates, by electrophoresis of DNA fragments which resulted in the digestion of the PCR product with Mnll, and by PCR-direct sequencing. The haplotype of the /3-globin-like gene cluster was determined by the presence (+) or absence (-) of the restriction enzyme site in the PCR product including the polymorphic site. Polymorphism or the haplotype were determined at seven sites, 5' to the ｣>gene//JmcII, in the ｰ7-gene/ifmdIII, in the Ay-gene/#mdIII, in the
