Serotyping of aChlamydia trachomatis strain by immunoblot analysis of the seorvar-specific major outer membrane protein (MOMP) antibody *
This paper presents the design and results of serotyping of a Chlamydia trachomatis strain by immunoblot analysis of major outer membrane protein (MOMP). The molecular weight of MOMP for the Tawma strain was 45kDa and that for the L2 serotype was 42kDa. The molecular weight of MOMP for A, C, D, H, I, J, K and the L3 serotype is known to be larger than that for L2. Antisera of C complex (Anti-A,C,I,J,K and L3) appeared to react more strongly against Tawma MOMP than L2 MOMP, and anti-D (B complex) serum appeared to react more strongly against L2 MOMP than Tawma MOMP. We devised an index for expressing the specificity to Tawma MOMP antibody as the antibody titer against Tawma MOMP/the antibody titer against L2 MOMP. The index of anti-C serotype serum was the highest among C complex antisera. Therefore it was decided that the Tawma strain was of the C serotype. The results achieved with our typing method were the same as those gotten with the immunotyping method using a monoclonal kit. (Acccpted on January 12, 2001) Kawasaki Igakkaishi 27(2) : 91-95. 2001
