Induction of cell death by cis-diamminedichlorplatinum (II) in a human testicular seminoma cell line (JKT-1)*
A human testicular seminoma cell line was treated with cis-diamminedichloroplatinum (II) (CDDP). Treatment with CDDP resulted in rapid and concentration-dependent cell death or decrease in cell numbers, confirming that CDDP has a strong anticancer activity against seminoma cells. The tumor cell nuclei showed fragmentation that was more pronounced at a high CDDP concentration. Cell cycle analysis after CDDP treatment showed that cells accumulated in the S and G2 /M phases, and that there was an increase in the number of hypoploid cells after appoximately 12 hours . The hypoploid cells were thought to have undergone apoptosis. Demonstration of DNA fragmentation by electrophoresis confirmed that CDDP caused the death of seminoma cells by inducing apoptosis. (Accepted on May 24, 1997) Kawasaki Igakkaishi 23(1) 35―43, 1997
