Characterization of DNA-binding of the Rat Uterine Estrogen Receptor
We characterized DNA-binding of the estrogen receptor from rat uterus by UV cross-linking and gel retardation analysis. The nuclear estriol- and LY139478 - receptor complexes from rat uterus were bound as a dimer as well as the other liganded nuclear receptors with a synthetic oligonucleotide containing a Xenopus vitellogenin A2 estrogen response element (VRE) which was substituted with 5-bromo-2'-deoxyuridine by UV irradiation. In addition, the nuclear antiestrogen-receptor complexes showed much higher binding ability with VRE than the nuclear estrogen-receptor complex under a high salt condition by gel retardation analysis.
