Determination of the Optimal Conditions for Flow Cytometric Analysis by Simultaneous Staining with Bromodeoxyuridine (BrdU) and Propidium Iodide (PI) in Isolated Nuclei of BG-1 Human Ovarian Cancer Cells
This study was undertaken to determine the optimal conditions for flow cytometric analysis of isolated nuclei by simulutaneous staining with bromodeoxyuridine (BrdU) and propidium iodide (PI) particularly in BG-1 human ovarian cancer cells. The following results were obtained: (1) The optimal concentration of BrdU to label the isolated nuclei of the BG-1 cells was 20 μM. (2) The optimal concentrations and times for HC1 treatment were 2 N for 20 min, 2.5 N for 15 min, and 2.5 N for 20 min.
