Two-Color Analysis of Epidermal Cell Suspension of Mouse with Anti-DNP and Anti-Thy-1 Antibodies after Skin Painting with DNCB
The epidermal cell suspensions prepared from DNCB painted ear skin of C3H/He mice were double-stained for Thy-1 protein and DNP groups. The cells with both Thy-1 alloantigen and DNP groups in the specimen treated with anti-DNP and followed by anti-Thy-1,2 incubation were detected more frequently than the cells treated with the antibodies in the reverse order. This suggests that DNCB is coupled in vivo to the Thy-1 alloantigen on the surface of Thy-1 positive cells.
