MONOCYTIC PROPERTIES OF FOUR LYMPHOBLASTOID CELL LINES ESTABLISHED FROM THREE PATIENTS WITH NON-LYMPHOCYTIC LEUKEMIA
Four human hematopoietic cell lines, designated as K-21, K-22, K-23-L and K-23-M, were established from three patients with nonlymphocytic leukemia. All of them were morphologically "lympho-blastoid cell lines". But these cell lines had properties like the monocyte or macrophage, such as the presence of Fc receptor sometimes up to 97% (K-23-M), positive stainability of α-naphthyl butyrate esterase that was blocked by NaF, and ability to migrate on soft agar, to attach to glass surface, and to phagocytize certain particles, polystyrene latex particles, opsonized or glutaraldehyde-treated sheep red cells. Among these monocytic properties examined so far in these 4 cell lines, the phagocytic activity of the K-23-M was the most remarkable finding. Consequently, these observations raise a question on the theory that lymphoblastoid cell lines originated from B-lymphocyte.
