THE MICROFLUORIMETRIC AND FLUORESCENCE MICROSCOPIC ESTIMATION OF THE ANTIGENICITY OF CELL MEMBRANES IN TWO HUMAN LYMPHOBLASTOID CELL LINES
By the microfluorimetry an attempt was made to estimate the results of staining with FITC-labelled antibody, according to the fluorescence strength emitted from isolated cells stained with indirect immunofluorescent method. Two human lymphoblastoid cell lines established from peripheral blood of different donors were used, and it could be known that the antigenicity of the cell membrane of both cells is somewhat different, owing partly to the alloantigen and partly to some other factors, e.g., the modification of cell membrane with viral infection, and that the usage of microscopic observation of samples under fluorescence microscope and microfluorimetry at the same time is very useful to estimate the results objectively.
