PROPERTIES OF ERYTHROCYTE MEMBRANE IN A HYPOTONIC MILIEU OF DECREASING OSMOLARITY (COIL PLANET CENTRIFUGATION)
A column of aqueous sodium chloride solution having a rectilinear osmotic gradient (150→30mOsM) was set on a coil planet centrifuge (CPC), which was filled with 10μl blood (which was inhibited from coagulation with Anticlot Et, and was used just after the treatment or after allowing to stand at room temperature for 4-6 hours); both ends of the column were sealed by heat-fusing, and after heating at 37℃ for 10 minutes, it was centrifuged by CPC (37℃ for 10 minutes) to observe the membrane properties of erythrocytes in a dynamic state with continuous decreasing of the osmotic pressure. One hundred normal subjects (49 males and 51 females) were examnied by this method and the following values and hemolysis band were obtained. Hemolysis starting point (HSP): 98.7 ±SD 5 mOsM Hemolysis end point (HEP): 63.8±SD 4 mOsM Hemolysis band shape: L type (sometimes M type) Thus, there may be employed HSP 100 mOsM and HEP 65 mOsM as the approximate standard values, and L type may be considered to be a standard from of the hemolysis band. Seasonal variation was seldom observed, and was slight, if any.
