Plasma ativity of the renin-angiotensin-aldosterone system, renal peroxidation, and effects of edaravone on suppressing peroxidation in puromycin-aminonucleoside-induced nephritis *
I investigated plasma activity of the renin-angiotensin-aldosterone（RAA） system, renal peroxidation, and effects of edaravone（EDA）on suppressing peroxidation in puromycin-aminonucleoside（PAN）-induced nephrosis. Thirty 8-week-old Wister male rats were used in this study. The rats were equally divided into three groups, and were intraperitoneally administered physiological saline（Control group）, PAN（PAN group）, and PAN and EDA（EDA group）, respectively. Blood pressure, serum and urinary biochemicals as well as plasma renin, angiotensin Ⅰ and Ⅱ , and aldosterone concentrations were measured. Distributions of nitric oxide（NO）and reactive oxygen species in renal tissues were observed after staining by using confocal laser-scanning microscopy. Plasma renin, angiotensin Ⅰ and Ⅱ, and aldosterone concentrations in the PAN group were significantly higher than those in the Control group. Plasma angiotensin Ⅰ and Ⅱ concentrations in the EDA group were significantly lower than those in the PAN group. Fluorescent brightness indicating NO production in glomerulus was significantly increased in the PAN group compared with that in the Control group. Fluorescent brightness indicating reactive oxygen species production in glomerulus was significantly increased in the PAN group compared with those in the Control and the EDA group. These results indicate that the plasma activity of the RAA system, and glomerular NO production and peroxidation are increased in PAN-induced nephrosis, and suggest that EDA has effects to suppress both increases in the plasma activity of the RAA system and the glomerular peroxidation. (Accepted on October 21, 2009)
