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Online edition:ISSN 2758-089X

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Characterization of DNA-binding of the Rat Uterine Estrogen Receptor

We characterized DNA-binding of the estrogen receptor from rat uterus by UV cross-linking and gel retardation analysis. The nuclear estriol- and LY139478 - receptor complexes from rat uterus were bound as a dimer as well as the other liganded nuclear receptors with a synthetic oligonucleotide containing a Xenopus vitellogenin A2 estrogen response element (VRE) which was substituted with 5-bromo-2'-deoxyuridine by UV irradiation. In addition, the nuclear antiestrogen-receptor complexes showed much higher binding ability with VRE than the nuclear estrogen-receptor complex under a high salt condition by gel retardation analysis.

著者名
Ikeda M, et al
23
3.4
117-122
DOI
10.11482/KMJ-E23(3.4)117-122.1997.pdf

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