Transdominant Negative FGF Receptors Inhibit Proliferation of Cultured Glomerular Mesangial Cells
Following detection of expression of FGF2 and FGF receptors in cultured rat mesangial cells, the inhibiting effects on proliferation of the cultured mesangial cells by transfection of kinase domain-deficient FGFR - 1 and FGFR - 2 were examined. Cultured rat mesangial cells expressed the FGF2, FGFR-1 and FGFR-2 genes. After 48 hrs of incubation, rh-FGF2 (10 ng/ml) increased the number of Alp-transfected rat mesangial cells to 1.8±0.3 fold that in nonstimulated cells. In contrast, transfection of dominant negative-FGFRs led to a significant inhibition of cell proliferation (p<0.001). Our results suggest that the proliferation of mesangial cells is stimulated by FGF2 and reversed by these dominant negative receptors. And FGFR-1 has a much more important role for mesangial cell proliferation than FGFR-2.