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Online edition:ISSN 2758-089X

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Use of Pronase and Pepsin in the Restoration of Laminin Antigenicity

To improve the restoration of laminin antigenicity, we applied pronase E and pepsin before immunohistochemical staining. In changing the concentration and reaction times of these enzymes, we found that the best results were obtained in sections treated with a 120 min pepsin reaction followed by 5 to 10 min pronase E treatment. Constant and consistent results were obtained with this procedure, and it is expected to improve the immunohistochemical study of laminin.

著者名
Yoshida K, et al
19
3.4
101-105
DOI
10.11482/KMJ19(3.4)101-105.1993.pdf

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