Histochemical and Ultrastructural Study of Clara Cell Granules in the Guinea Pig
Histochemical studies using light and electron microscopes were undertaken to see what chemical components make up the Clara cell granules in guinea pig lung. Light microscopically, it was suggested that they may contain a variable amount of proteins, carbohydrates, and phospholipids. On the other hand, electron microscopic examination with digestive treatment with pepsin as well as chloroform-methanol clearly demonstrated that the main component of the crystalline granules of the guinea pig Clara cells was the proteins. It was, therefore, concluded that Clara cell granules are complex compounds made up mainly of proteins with small amounts of carbohydrates and phospholipids.
