Steroidogenesis in Isolated Adrenal Cells of Rat
A reliable and reproducible system for the isolation of rat adrenal cells was developed, using 0.25% trypsin for cell dispersion. The suspending cell in Krebs-Ringer bicarbonate buffer containing 0.2% glucose and 0.5% bovine serum albumin was incubated for 120 minutes at 37℃ under 95% 02 and 5% C02. Corticosterone production induced by synthetic 1-24ACTH showed a dose-related increase in decapsular cells. The precision of the inter-experiment of corticosterone production was 5.0% (average coefficients of variation).
