Antigen in Contact Sensitivity : I. Immunofluorescent Studies on the Distribution of Bound DNCB within the Epidermis of Guinea Pigs Following Skin Painting with the Sensitizer
Cryostat sections, sheet and single cells of epidermis prepared from ear skin of guinea pigs 3 hours after painting with 5% DNCB ethanol solution were examined by using FITC labelled anti-DNP antibody. DNP groups were demonstrated on almost 100% of Malpigian cells of the epidermis, partial distribution of them was seen in horny layer. The number of DNP cells in the single cells was 80.2% on an average and roughly the same as the cells incubated in vitro with 30mM solution of DNBS03Na. Significance of these findings is discussed.