Assessment of Macrophage-neutrophil Interaction in the Delayed Type Hypersensitivity Reaction of Guinea Pigs
Electron microscopic observations were made of exudate macrophages with phagocytosed autologous neutrophils in intraperitoneally implanted collagen sponges in sensitized and non-sensitized guinea pigs. In addition, the number of these macrophages per unit area of sponge was calculated. Of the mononuclear cells, 12.7% were such macrophages in the sensitized group and 4.9% in the non-sensitized group, 48 hours after implantation. Macrophages with ingested neutrophils or cell debris and ferritin as the antigen in cytoplasmic phagolysosomes often formed clusters with a lymphocyte series in the sensitized guinea pigs, but not in the non-sensitized guinea pigs.