DETERMINATION OF PLASMA ANTIEPILEPTIC DRUGS BY A NEW KIT (MARKIT) BASED ON A COMPETITIVE ENZYME-IMMUNOASSAY
Three kinds of antiepileptic drugs, phenytoin (DPH), phenobarbital (PB) and primidone (PM), in plasma samples from patients with epilepsy were measured by a new technique "MARKIT" based on a competitive binding enzyme-immunoassay, and the obtained values were compared with these by the other method-EMIT based on homogeneous enzyme-immunoassay. Correlative coefficient of the values between MARKIT (x) and EMIT (y) was r=0.980, n=85 for DPH ; r=0.978, n=117, for PB ; and r = 0.978, n=66, for PM. The equation of a regression line of the obtained values was close to y = x, with all three drugs. MARKIT has a merit to be applicable to routine apparatuses used in the ordinary clinical laboratories. It requires about two hours for one sample assay, but only 3-5 minutes per one sample when it comes to simultaneous assays for 50-100 samples.