FREEZE-FRACTURE STUDY OF THE RAT LUNG
The lamellar inclusion body of the rat alveolar type II cell was studied using freeze-fracture replication technique without chemical fixation or glycerinization. Ripple marks with 18-20 nm spacing were observed in occasion. Such marks were considered to be a product of artefact, resulting from rearrangement of phospholipid molecules of the lamellae during rapid freezing.