DEMONSTRATION OF DNP GROUPS ON DRAINING LYMPH NODE CELLS IN GUINEA PIGS FOLLOWING SKIN PAINTING WITH DNCB BY SCANNING IMMUNOELECTRON MICROSCOPIC METHOD
The distribution of DNP groups on the cells taken from the draining lymph nodes of guinea pigs 12 hours after painting skin with DNCB was examined by the indirect method of scanning immunoelectron microscopy using bacteriophage T4 labelled with antibody as visual marker. Fairly precise localization of DNP groups on the surface of the cells could be followed by the method. It was indicated that DNP groups were distributed in various amounts and in various patterns (diffuse, patchy or sparse) on the surface of the cells in the draining lymph nodes of animals when painted with DNCB. The incidence of the cells detected DNP groups on their surface was approximately 58%. The frequency of the cells with DNP groups was markedly reduced in the animals treated with DNBSO8Na as compared to animals not receiving the tolerogen. The significance of these findings is discussed.