SPIRONOLACTONE BODIES : A STUDY WITH TRANSMISSION ELECTRON MICROSCOPE AND FREEZE-FRACTURE REPLICATION
A treatment with Aldosterone antagonist, Spironolactone, is known to produce acidophilic cytoplasmic inclusions in the zona glomerulosa cells of adrenal corteces. Transmission electron microscopic study disclosed that the inclusions were composed of concentric whorls of membranes and/or a regular aggregation of tubules, devoid of limiting membrane. The formalin-fixed tissue seemed to be an adequate material for freeze-fracture study. Freeze-fracture replication study of the inclusion body confirmed that the inclusions lacked limiting membranes and each concentric lamellae were comprised of smooth membranes with occasional membrane-associated particles. It is speculated that spironolactone body may be a partially degenerated aggregate of the smooth endoplasmic reticulum, overgrown through either direct or indirect stimulation by aldosterone antagonist.