FREEZE-FRACTURE STUDY OF THE RAT LUNG I. GENERAL DESCRIPTION OF RAT ALVEOLAR CELLS IN THE GLUTARALDEHYDE-FIXED AND GLYCERINIZED LUNG TISSUE
There is no established standard procedure in processing tissue for freeze-etching or freeze-fracture replication. A review of the literature reveals that specimens suitable and convenient for such study may be obtained from glutaraldehyde-fixed and glycerinized tissue. In this experiment we have undertaken to describe completely the morphological appearance of alveolar type Ⅱ cells as well as a few other cells in rat lung, after fixation in glutaraldehyde for three hours and glycerinization for one hour before freeze-fracture replication. It is expected that this will provide a standard of reference when structural features of rat lung cells in tissues processed by different procedures are compared.