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Online edition:ISSN 2758-089X

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IDENTIFICATION OF GUINEA PIG T LYMPHOCYTES ON FROZEN TISSUE SECTIONS BY ROSETTE FORMING ASSAY

Guinea pig T lymphocytes were identified on fronzen tissue sections by rosette forming assay, in which neuraminidase-treated rabbit erythrocytes (En) were used as the indicator cells. This assay was undertaken by humidity chamber technique. Rabbit En adhered to the white pulp of the normal spleen where T lymphocytes occupy preferentially, and in the case of allografted skin rabbit En adhered mostly to the epithelial region after 4 and 5 days in guinea pigs. The characteristic adherence of rabbit En to guinea pig blood T lymphocytes in suspension was observed by scanning electron microscopy (SEM).

著者名
Nakayama Y, et al
4
4
265-270
DOI
10.11482/KMJ-E4(4)265

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