STUDY ON THE IN VIVO AND IN VITRO REACTIVITY OF GUINEA PIG SKIN WITH DINITROBENZENE COMPOUNDS
The distribution of DNP groups in guinea pig skin following surface application of DNCB in various solvents was investigated by immunofluorescent and immunohistochemical methods. There was no fundamental difference in the distribution by varying the solvents. DNP groups were detected in horny and Malpigian layers, but not in the so-called transition layer and collagen bundles. In vitro, the affiinity between DNCB and either epidermis except transition layer or collagen bundles was demonstrated. The significance of these findings is discussed.