RADIOIMMUNOASSAY FOR STEROID HORMONES Ⅲ. RADIOIMMUNOASSAY FOR PLASMA DEHYDROEPIANDROSTERONE
A specific, sensitive and reliable radioimmunoassay for plasma dehydroepiandrosterone (DHEA) has been developed. Anti-DHEA serum was obtained by immunizing rabbits with a DHEA-3-hemisuccinate-BSA conjugate. A useful range in the standard curve was from 10 pg to 500 pg. DHEA was separated from cross-reacting steroids by microcolumn chromatography. The coefficients of variation for within-assay and between-assay are 6.3 % and 7.7 %, respectively.