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Online edition:ISSN 2758-089X

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RADIOIMMUNOASSAY FOR STEROID HORMONES Ⅲ. RADIOIMMUNOASSAY FOR PLASMA DEHYDROEPIANDROSTERONE

A specific, sensitive and reliable radioimmunoassay for plasma dehydroepiandrosterone (DHEA) has been developed. Anti-DHEA serum was obtained by immunizing rabbits with a DHEA-3-hemisuccinate-BSA conjugate. A useful range in the standard curve was from 10 pg to 500 pg. DHEA was separated from cross-reacting steroids by microcolumn chromatography. The coefficients of variation for within-assay and between-assay are 6.3 % and 7.7 %, respectively.

著者名
Nisida S, et al
3
3
181-186
DOI
10.11482/KMJ-E3(3)181

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