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Online edition:ISSN 2758-089X

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RADIOIMMUNOASSAY FOR STEROID HORMONES Ⅳ. RADIOIMMUNOASSAY FOR PLASMA PREGNENOLONE

A convenient and reliable radioimmunoassay for plasma pregnenolone has been developed. Anti-pregnenolone serum was obtained by immunizing rabbits with a pregnenolone-3-hemisuccinate-BSA conjugate. A useful range in the standard curve was from 10 pg to 500 pg. Pregnenolone in 0.1 ml plasma was extracted with ether and was separated from cross-reacting steroids by microcolumn chromatography. Plasma concentrations of pregnenolone at 9 a.m. were: men, 0.91±0.17 ng/ml; women, 1.06±0.37 ng/ml.

著者名
Nishida S, et al
3
3
219-225
DOI
10.11482/KMJ-E3(3)219

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