h_kaishi
Online edition:ISSN 2758-089X

t_toukou

SERUM LDH5 ISOZYME DETERMINATION BY A COLUMN METHOD (“CHEMIPHAR” TEST)

A LDH isozyme assay by column chromatography ("Chemiphar" kit) was compared with the conventional electrophoretic method, and its usefulness as a routine procedure for estimation of LDH5 isozyme was recognized. It was simple in manipulation, requires short time for getting the result and gave satisfactorily precise estimations of LDH5 (coefficient of variation was 7.3 %), It showed a good correlation with the agar gel electrophoretic method (correlation coefficient, r=0.93), but gave values slightly higher than those obtained by electrophoresis on account of contamination of the LDH5 effluent from the column with minute amount of LDH4 isozyme. However, this is insignificant for exact interpretation of the estimations. In this method the coloration for estimation of LDH isozyme was successfully stabilized by use of Merudola Blue as hydrogen acceptor instead of the conventional Phenazine Methosulfate. This column ("Chemiphar") method is recommended for screening the sera with abnormal LDH5 isozyme activity.

著者名
Ueda S, et al
3
4
287-294
DOI
10.11482/KMJ-E3(4)287

b_download