STUDIES ON UPTAKE OF FATTY ACIDS INTO MYCOBACTERIAL CELLS (I) EFFECTS OF p-CHLOROPHENOXYISOBUTYRATE ON THE UPTAKE OF ACETATE INTO THE CELLS
It was demonstrated that p-chlorophenoxyisobutyrate (CPIB), a potent hypolipidemic agent, has several powerful anti-biological activities against Mycobacterium smegmatis, such as growth inhibition, inhibition against uptaking of acetate and decreasing of ATP's content in the cells. On the other hand, 4-pentenoate, an effective reagent for reducing free CoA's concentration in living organisms, was found to inhibit also the acetate-uptake of the cells. From these results obtained, it could be imagined that acetyl CoA synthetase reaction is involved in the uptake of acetate into the cells. Occurence of at least two inhibitory steps of CPIB against biosynthesis of lipids in this micro-organisms in vivo was postulated, also.