A protocol to assay Anti-Yersinia pseudotuberculosis-derived mitogen human IgG by ELISA
Our department has been continuing a research on the diagnosis of Yersinia pseudotuberculosis infections. This pathogen manifests in multifarious forms, including systemic vasculitis resembling Kawasaki Disease. Recently, a previously established ELISA assay for this infection was transferred from its originator at National Center for Child Health and Development. However, the protocol had to be modified for a different facility and equipment. Described herein is the protocol, successfully adapted for use at our laboratory. This protocol is contributing to the aforementioned research by improving analysis throughput of our laboratory.
